Metabolomics:
The Institute of Molecular Biology and Biotechnologies offers to perform original and targeted LC-Mass based metabolomics analyses for different compounds in plants, animals and humans for diverse clients. While plants methods are developed for the resurrection plant Haberlea rhodopensis Friv. With which we have substantial experience, they can still be applied to plants with a high burden of phenolic substances, as well as to different various other plants. We also offer LC-Mass targeted investigations for biologically active compounds in medical plants and high-value bioactive commodities (carotenoids, anthocyanin, flavonoids etc.) from agriculture plants.
- Workflow strategy for untargeted LC-hrMS/MS metabolomics in plants. A single platform will be used for complete metabolomics, eliminating the need of GC-MS and Ion chromatography.
- Targeted study of nicotine and nicotine glucuronide as a component element or a product of decomposition
- Targeted analysis of amino acids (>30) and small peptides in plant tissue. An economical, practical, fast and robust method for analysis of over 28 amino acids and up to 100 amino acid derivatives.
- Targeted analysis of Nucleosides, Nucleotides and Nucleobases (32 individual representatives). A simple fast and robust method for nucleobase, Nucleotide, nucleoside and their phosphate form analysis was developed. All samples are processed in a single run with the new HILIC technology.
- Targeted analysis for sugars in plant tissue (from 1 to 10 sugar units). A single run can cover anything from monosaccharides to 15 monomer oligo sugars. Separation of isobars.
- Targeted and untargeted analysis of saponines in plant tissue, pharmaceutical forms and crude extracts. Ultrafast, ultra-informative, ultrasensitive, ultra-robust method using amidHILIC technology.
- Analysis of steroidal hormone levels in blood serum and plant tissue.
- Analysis of vitamin levels in blood serum and plant tissue (a single analysis for water and fat-soluble vitamins).
- Combined single analysis workflow for untargeted and targeted metabolomics and proteomics, incorporating innovative fractionation technology, along with both Information-dependent and Information-independent instrumental setups for complete sample examination.